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FEMTO Pulse™

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Advances in molecular biology are driving life science research forward at an unprecedented rate. Progress is often inhibited by slow and outdated methods of analysis that have not kept up with this breakneck speed. The FEMTO Pulse is a revolutionary instrument for the analysis of nucleic acids. Capable of detecting down to 1 fg/µl of sample and accurately sizing fragments and smears through 165,000 bp, the FEMTO Pulse is breaking all the QC barriers.
The enhanced sizing capability of the FEMTO Pulse is the direct result of incorporating a pulsed-field power supply into the instrument. Pulsed-field electrophoresis is a powerful separation method and a proven way to separate very large DNA fragments and smears. On the FEMTO Pulse, three factors are controlled to provide high quality separations of large nucleic acid fragments and smears:

  • Voltage polarity

  • Oscillation frequency

  • Wave form of the oscillation frequency

The FEMTO Pulse has all the separation advantages of pulsed-field electrophoresis, but is able to complete a separation 15-20X faster than the current method while automating the analysis.

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The high sensitivity of the FEMTO Pulse is the result of a combination of several factors, including:

  • Optimized optical sensors

  • Improved illumination and excitation at the detection windows of the intercalation dye

  • Redesigned algorithms that facilitate improved detection

  • Improved gel chemistries

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The combination of femtogram level sensitivity and accurate sizing of very large nucleic acid fragments and smears allows the FEMTO Pulse to break analytic barriers.

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