Patented 96-capillary array allows simultaneous injection of up to 96 individual samples from a standard PCR plate.
Analyzing DNA fragments is a critical but often tedious aspect due to manual data transcription methods. Now there is an automated technology through parallel capillary electrophoresis to solve these issues. The ZAG™ DNA Analyzer uses a patented capillary array to simultaneously inject up to 96 individual samples from a standard PCR plate. Capillaries are arranged into a tight formation and illuminated with a single high-powered LED light source. All of the DNA fragments moving through the capillaries are bound with intercalating dye, emitting a fluorescent signal detectable to the CCD camera and captured by a data management system. Post electrophoresis, analytical software is used to generate numerical results.
First, fragment size can be determined by the time required to pass through the detection window. Second, the nucleic acid concentration can be determined by the relative emission signal strength when compared to a calibrated ladder. And third, fragments can be flagged and analyzed for report generation. By repriming each capillary with fresh separation gel matrix prior to each electrophoresis cycle, the ZAG DNA Analyzer offers high throughput analysis with virtually no failure rate.