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Technology

Parallel capillary electrophoresis (CE) enables small— to large—volume electro—driven analytical separations of DNA, RNA and gDNA, with fluorescent detection.

Our platform allows you to achieve the perfect balance of speed and resolution by altering capillary length. Longer arrays can take resolution down to 2 bp for fragments under 300 bp in length. Shorter arrays will get through runs in as little as 15 minutes for certain applications.

The inlet side of the capillary array is arranged in an 8 x 12 format, with electrodes next to each capillary. This allows direct injection via vacuum or voltage from standard 96-well plates. The capillaries are aligned in parallel in the detection window, which is positioned between the LED light source and the detector assembly. The outlets of the capillaries are bundled to a common reservoir, where they are connected to a ground. The capillary arrays are modular and can be exchanged in less than five minutes.

Room temperature air is circulated around the capillaries to modulate the temperature during CE separation.

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Technology configuration

The optical platform consists of an LED light source (470nm excitation), camera lens and CCD detector. Fluorescent light from the LED passes through the array window, exciting the intercalating fluorescent dye in the gel matrices. This is captured by the camera lens and spatially imaged onto the CCD detector. Imaging is constant, so you get real time, on-line detection of separations.

The power supply provides appropriate voltage for electrokinetic sample injection and electrophoresis. The recommended operating limits are +/-16 kV, 3 mA total current (30µA per capillary).

A syringe pump provides controlled pressure or vacuum to the outlet reservoir of the capillary array. Vacuum injection can be especially useful for eliminating the effects of salt on nucleic acid uptake.

Automation

Built-in software automates capillary conditioning, sample injection, electrophoresis separation and data processing. You can visualize results in your preferred format and share reports easily.

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