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DETECT DNA FRAGMENTS DOWN TO 5 fg/µL
A serial dilution of a 300 bp fragment was electrophoresed under standard conditions for this application, depicted in the accompanying electropherogram and gel image. Final fragment concentrations used for this study are 39 fg/µL (black trace, lane 1), 19.5 fg/µL (red trace, lane 2), 9.7 fg/µL (blue trace, lane 3), 4.8 fg/µL (orange trace, lane 4). In the gel image, the ladder in shown in lane 5. The lowest concentration represents approximately 15,000 molecules of the 300 bp fragment.
SEPARATE AND DETECT A SINGLE CELL’S WORTH OF GENOMIC DNA
In 10 µL of a diluent solution, 16 pg (red) and 8 pg (black) of high molecular weight genomic DNA was diluted and separated using pulsed-field electrophoresis conditions. Final concentrations of each genomic sample used in this study are 1.6 pg/µL (red trace) and 0.8 fg/µL (black trace). The superior sensitivity and resolution of the FEMTO Pulse sized the single cells genomic content to approximately 175,000 bp.
TOTAL RNA SEPARATION FROM A SINGLE CELL
Total RNA was extracted from mouse cells and separated under standard conditions for this application. In this example, 2 µL of total RNA at 5 pg/µL was mixed with 18 µL of a diluent marker solution for a final concentration of 500 fg/µL. Under these conditions, this represents approximately a single cell’s worth of total RNA. The electropherogram displays the high quality RNA without degradation products. A high RQN value is calculated for this sample and shown in the call out box.