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FEMTO Pulse Automated Pulsed-Field CE Systlem
REVOLUTIONIZE YOUR NUCLEIC ACID ANALYSIS
The FEMTO Pulse™ Automated Pulsed-Field CE Instrument is revolutionizing nucleic acid analysis. Combining the automation of capillary electrophoresis with the control of pulsed-field power, it is the only automated pulsed-field capillary electrophoresis instrument on the market.
Through an advanced instrument design, large molecular weight DNA fragments can be separated and nucleic acids detected into the femtogram range. Compared to other non-denaturing gel-based analytical instruments, the FEMTO Pulse easily achieves 20 to 30 times higher sensitivity for nucleic acid smears and up to 100 times higher sensitivity for nucleic acid fragments.
Applications for the FEMTO Pulse include analysis of single cell total RNA or genomic DNA, low quantity NGS library preparations (PCR-free), exosomes, small RNA, cfDNA, FFPE nucleic acid isolates, large DNA fragment libraries, BAC clones, foreign DNA in vaccine preparations, and single DNA or multiple DNA fragments from low concentration samples.
With all the changes that are happening in genomics discovery, the FEMTO Pulse is pushing the boundaries of science to enable discovery. What will you discover with a more sensitive instrument?
PULSED-FIELD POWER FOR FAST, LARGE FRAGMENT SEPARATIONS
Pulsed-field electrophoresis is a powerful separation method and a proven way to separate large DNA fragments. By using a pulsed-field power supply on the FEMTO Pulse, DNA smears as large as 165,000 bp can be separated in just one hour while DNA fragments, as large as 200,000 bp can be separated in under two hours. Separations are directed by four factors including: 1. Alternating the voltage polarity from negative to positive 2. Regulating the oscillation frequency 3. Controlling the timing or ramping of the oscillation frequency 4. Governing the oscillation wave form
To achieve the femtogram levels of sensitivity, the entire excitation and detection system on the FEMTO Pulse™ was redesigned.
• Proprietary changes were made to the way the nucleic acids are detected.
• The power density was increased on the detection window for heightened illumination and excitation
• New software was developed to measure and record the nucleic acids as they pass the detectors.
• Specialized gel chemistries were created to ensure the lowest possible background noise
DETECT DNA FRAGMENTS DOWN TO 5 fg/µL
A serial dilution of a 300 bp fragment was electrophoresed under standard conditions for this application, depicted in the accompanying electropherogram and gel image. Final fragment concentrations used for this study are 39 fg/µL (black trace, lane 1), 19.5 fg/µL (red trace, lane 2), 9.7 fg/µL (blue trace, lane 3), 4.8 fg/µL (orange trace, lane 4). In the gel image, the ladder in shown in lane 5. The lowest concentration represents approximately 15,000 molecules of the 300 bp fragment.