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Small RNA Analysis Kit
제니어 조회수:500 180.66.101.94
2016-10-31 21:46:47

 

 

The Small RNA Analysis Kit provides automated analysis of small RNAs. The kit excels in the evaluation of small RNAs for downstream applications, notably including small RNA NGS libraries. Able to distinguish between precursor small RNA and functional small RNA, it can also quantify the amount of microRNA present in any given sample. To help streamline laboratory workflows, post-separation analysis using the Small RNA Analysis Region function in PROSize® 2.0 easily quantifies the microRNA and small RNA present in a sample. Note: This kit is optimized for use on a 12 capillary array.

 

Examples

With the increasing interest in small RNAs for downstream applications such as NGS, the accurate and efficient analysis of small RNAs from a variety of sources is crucial. Small RNA sample analysis is dependent upon the employment of a high quality small RNA ladder as seen in Figure 1. The Small RNA Analysis Kit (DNF-470) is able to measure the size and concentration of small RNAs, including functional microRNAs, due to the Small RNA Ladder (DNF-366). The Small RNA Ladder, comprised of small RNA fragments, enhances the accuracy and precision of fragment size and concentration measurements beyond other analysis methods.

 

Figure 1.A typical separation of the Small RNA Ladder (DNF-366) performed on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the Small RNA Analysis Kit (DNF-470)

 

The Small RNA Analysis Kit (DNF-470) provides a more detailed analysis of the small RNA region than possible with the HS Total RNA Analysis Kit (DNF-472) as shown in Figure 2. The same total RNA sample from Corn Leaf was run on the Fragment Analyzer using each respective analysis kit. The HS Total RNA Analysis Kit (DNF-472) provides an excellent overview of the sample, indicating that the extracted total RNA is high quality with an RQN of 10.0. The Small RNA Analysis Kit (DNF-470) takes the analysis a step further with not only a more detailed separation of the small RNA region, but quantifying the amount of small RNA and microRNA present in the total RNA sample.

Figure 2. Total RNA extracted from corn leaf separated using the High Sensitivity RNA Analysis Kit (15nt) (DNF-472) (TOP) and the Small RNA AnalysisKit (DNF-470) (BOTTOM). Capillary electrophoresis was performed on a Fragment Analyzer equipped with a Short Capillary Array (33-55). Both kits show the small RNA region, but the Small RNA Analysis Kit (DNF-470) depicts the region in more detail, distinguishing between the microRNA region (10nt - 40nt) and the small RNA region (10nt-200nt).

 

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