Notice & News
The High Sensitivity NGS Fragment Analysis Kit provides an automated solution to the quality assessment of low concentration NGS libraries prior to sequencing. The correct determination of molarity for optimum flow-cell loading is a critical step in NGS, and DNF-474 efficiently and accurately determines the average size of a library and its concentration. The correct calculation of molarity helps ensure quality NGS results. Use DNF-474 to increase the efficiency of your genomic research.
In the following section we have provided several examples of typical and atypical separations performed with the HS NGS Fragment Analysis Kit on a Fragment Analyzer fitted with a standard short array.
Figure 1 depicts a typical separation of the HS NGS DNA Ladder (DNF-396). The peaks are clearly defined and well separated, providing a precise and accurate standard curve for the sizing and quantitation of samples.
Figure 1. A typicl separatinof the HS NGS Ladder (DNF-396) on a Fragment Analyzer equipped with a Short Array(33-55) using the HIgh Sensitivity NGS Fragment Analysis Kit (DNF-474)
Assessment of the size and quality of a library smear is important to obtaining quality sequencing results. Figure 2 shows a high quality library constructed from commercially prepared human genomic DNA.
Figure 1. A high quality NGS library. Salmples were run using the HS NGS Fragment Analysis Kit on a Fragment Analyzer equipped with a Short Capillary Array (33-55)
Importantly, the HS NGS Fragment Kit is able to detect primer dimers and adapter dimers, significant indicators of library quality. High levels of dimers, adapter or primer, interferes with sequencing, reducing the quality of the results. Figure 3 depicts a separation of an NGS library that has primer dimers and adapter dimers. Primer dimers typically appear around 70 bp, while adapter dimers appear around 140 bp.
Figure 3. An NGS library with primer and adapter dimers. Samples were run using the HS NGS Fragment Analysis Kit ona Fragment Analyzer equipped with an Ultra-Short Capillary Array(22-47)